GERC - Lisette Maddison
Center for Reproductive Biology
Gene Editing Reagent Core
Helping investigators integrate cutting edge genome manipulation into their research

Gene Editing Reagent Core

Directed by Dr. Lisette Maddison, the Gene Editing Reagent Core (GERC) provides a fee-based service to provide CRISPR/Cas9 reagents for genome manipulation in many species. 

Reagents will be developed in close consultation with the investigators for personalized results. Available services include individual guide RNA and Cas9 supplied separately or cloned into plasmid vectors and repair templates can be generated for precise genomic changes from small to large.

Advances in gene editing have made it the standard for functional genomics. The Gene Editing Reagent Core (GERC) is poised to assist investigators to incorporate gene editing into their research and offers fee-based services tailored to the needs of investigators. The GERC has successfully worked with investigators in a variety of ways, from generating plasmid-based systems for editing genes in cell lines, stand-alone reagents such as sgRNA and Cas9, to development of new approaches for editing in out-of-the-ordinary model systems. In conjunction with the WSU Animal Production Core, we also offer seamless development of new animal models, from design to delivery of the appropriately modified model. The GERC provides expertise in design and implementation of gene editing and services are tailored to be specific to the investigators and looks forward to working with anyone interested in incorporating genome editing into their research.

Contact

Services and costs

Please contact Dr. Lisette Maddison or call 509-335-8992 to obtain the most accurate pricing and approach for your project.

Or if you are interested in a service not listed here, we may be positioned to help.

Services cover several areas.

Our service, in conjunction with the Animal Production Core, is designed to be a seamless start to finish system to lessen the burden on the investigators. In close consultation with the investigators, we will design the approach, test that the approach will be successful using cultured embryos, make modifications where needed, then proceed to achieving a founder animal. Investigators can choose to receive the founder animal(s) or have us breed to the first generation.

  • CRISPR “knockouts”
    • Generating mutations or deletions leading to a non-functional or no protein
  • Locus deletion
    • Removal of large segments of the genome
  • Small changes
    • Including codon changes or addition of epitope tags
  • Large additions
    • Knock-in of larger elements such as Cre or fluorescent proteins to desired location, typically the 3’ end of a gene
  • ROSA knock-in
    • Targeting the ROSA locus for integration of transgenic constructs

Tools for editing cell lines/organisms of interest: Depending on goals these can be in different forms.

  • Plasmid based with antibody selection
    • Designed primarily for use in mammalian cell lines
  • Stand-alone Cas9 protein and sgRNA
  • Custom configurations suitable for less than ordinary research models
  • Constructs for virus-based gene editing
    • AAV or lentivirus
    • Note: We do not generate the viral particles

Screening of edited cell line/organisms: Ask us how we can help identify your edits.

Custom plasmids (we’re more than just CRISPR): Please contact us with your goals. 

Facilitated by the 10x Genomics Chromium platform, the single-cell library service allows interrogation of the transcriptome, epigenomic landscape, and/or immune cell diversity at single-cell resolution. Let us help elevate your research by incorporating state-of-the-art single-cell-omics.

Users submit single cell or nuclei suspensions, depending on the application and cell type, and we will return sequencing-ready libraries. We anticipate return of libraries within 7 working days of receipt of samples. Due to current delays in requisition processing by WSU Purchasing, we respectfully request a minimum of 4-week lead time in order to obtain the necessary reagents.

For assistance with scRNA-seq analysis pipeline, please contact Sarayut Winuthayanon.