Laboratory for Biotechnology and Bioanalysis (LBB)
Center for Reproductive Biology
Molecular Biology and Genomics Core
Providing individual investigators local access to state-of-the-art molecular biology and next generation DNA sequencing tools

Molecular Biology and Genomics Core

The Molecular Biology and Genomics Core, co-directed by Drs. Michael Griswold and Derek Pouchnik, provides DNA sequencing, protein sequencing, oligonucleotide synthesis, peptide synthesis, and some information services to members of the WSU Center for Reproductive Biology. The core is housed in the Laboratory for Biotechnology and Bioanalysis.

The core provides all genomics micro-array technology equipment, including access to an Affymetrix Micro-Array apparatus and data analysis system, as well as a custom array apparatus for glass slide array of investigator initiated arrays. It has the bio-informatics support for the procedures performed and assists the investigators in data analysis. Technical support is provided to perform the analysis on the Affymetrix chips and in the production of the custom arrays.

Services and instruments

  • Sanger DNA sequencing and genotyping (fragment analysis) on our ABI 3730
  • Two real-time PCR instruments (ABI 7500SDS) are available for qPCR and genotyping applications

The core also runs and maintains two next generation DNA sequencing platforms: the Ion Proton and the Pacific Biosciences Sequel. Library and sample preparation services for these platforms and data acquisition are offered. Some bioinformatics support is available for specific applications.

Resources

  • ABI 3730 DNA Analyzer
  • Agilent 5200 Fragment Analyzer
  • Applied Biosystems 7500fast Real Time PCR (x2)
  • Ion Proton, Life Technologies
  • PacBio Sequel, Pacific Biosciences
  • Takara High Throughput qPCR/Genotyping

Contact

Laboratory of Biotechnology & Bioanalysis
227 Biotechnology/Life Sciences Building
Pullman, WA 99164-7520
509-335-1174
lbb@wsu.edu

Lab hours
8 a.m. – 5 p.m. Monday – Friday

Cost of services
See pricing list