Monoclonal Antibody Center
Panels of monoclonal antibodies are available for a variety of species.
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Monoclonal Antibody Center

The Monoclonal Antibody Center provides a range of services related to the production and characterization of monoclonal antibodies to researchers and clinicians affiliated with WSU. 

Monoclonal antibodies to leukocyte differentiation molecules, activation molecules, and MHC Class I and II molecules in many species of food and companion animals have been produced by the WSU Monoclonal Antibody Center. 

Taxonomic Key Program is a searchable database that allows researchers to easily identify monoclonal antibody-defined molecules by their physical characteristics.

For prices and availability or technical questions, contact Victoria Hulubei, 509-335-6054, or Dr. Bill C. Davis, lab director, 509-335-6051.


Monoclonal antibody production services

Catalog #Description
MA2 Production of monoclonal antibodies: Immunization of mice, collection of pre-immune and immune sera, screening of sera by ELISA, cell fusion to produce mAb producing hybridomas, identification and cryo-preservation of mAb positive hybridomas.
MA3Cloning of hybridomas producing mAb of interest: Prepare and cryo-preserve 3 clones from each hybridoma cell line producing a mAb of interest.
MA4Production of 500 mg monoclonal antibody.
MA5Preparation of monoclonal antibody in serum free medium.

Isotype controls

Catalog #mAbIg isotypeE coli J5
COL2001COLIS69AIgG1E coli J5
COL2002COLIS205CIgG2aE coli J5
COL2003COLIS169AIgG2bE coli J5
COL2004COLIS41BIgG3E coli J5
COL2005COLIS52A2IgME coli J5

Monoclonal antibodies by species

MoAbIg isotypeSpecificity
H58AG2aMHC CL I
PT85AG2aMHC CL I
H42AG2aMHC CL II HLA-DP LIKE
TH14BG2aMHC CL II HLA-DR
TH81AG2aMHC CL II HLA-DQ
TH97AG2aCD1b
BAQ95AG1CD2
MUC2AG2a CD2
CACT31ACD2M
MM1AG1CD3
ILA-11AG2aCD4
GC50AMCD4
CACT138AG1CD4
BAQ91AG1CD6
BAQ83AG2bCD6
BAQ111AMCD8
CACT80CG1CD8α
7C2BG2aCD8α
BAT72AG1CD8β
LT86AG2aCD9
B18AG1CD9
RH1AG3CD9
RACT48AG2aCD11a
HUH73AG1CD11a
MM10AG2bCD11b
MM12AG1CD11b
BAQ153AMCD11c
CAM36AG1CD14 LPS receptor (monocytes, variable on granulocytes in different species)
CAM66AMCD14 LPS receptor (monocytes, variable on granulocytes in different species)
LND37AG1Monocytes
LND68AG1Monocytes
BAQ30AG1CD18
HUH82AG2aCD18
GB25AG1CD21
TE1AMCD28
FW4-101G1CD29
ILA158AG1CD40
CAPP2AG1CD41
Bag40AG3CD44
BAT31AG1CD44
LT36AG2bCD44
LT41AG2aCD44
LCT27AG1CD45R
GS5AG1CD45R
GC6AMCD45R
GC42AG1CD45R0
ILA116AG3CD45R0
TH17AMCD47
HUH71AG1CD47
BAQ92AG1CD62L
DH59BG1CD172a
LND41AG1DC and a subset of innate lymphoid cells
B7A1MWC1-N1
BAQ4AG1WC1-N2
CACTB32AG1WC1-N3
BAQ89AG1WC1-N4
ILA29AG1WC1
GB45AG1WC1-N26
GB54AG2aWC1-N25
GB21AG2bTCR1-N24 δ chain specific
CACTB6AMTCR1-N6 γ chain specific?
CACT22BG1TCR1-N7 γ chain specific?
86D1G1TCR1-N7 cluster, γ chain specific?
BIG73AG1sIgM
BIG715AG1IgG1
BIG501EG1λ light chain
BAQ44AMB cells
BAQ155AG1B cells
CH138AMGranulocytes
MM20AG1Granulocytes = CH138A
GS23AG1Neutrophils
CACT108AG2aCD25
CACT116AG1CD25
LCTB2AG3CD25
LCTB32AMCD25
CACT26AG2bCD26
KTSN7AG1CD69
ILA77AMCD71
CACT7AMACT1
CACT200AG1 ACT1
CACT206AG2aACT1
CACT26AG1ACT2
LCTB6AG1ACT9
GB110AMACT16
CACT195AM ACT27
CACT216AMACT28
CACT225AG1 ACT29
CACT152AM ACT31
CACT191AM ACT32
CACT180AG1ACT34
CACT282AMACT35
CACT153AMACT37
CACT243AMACT38
NK64AG1NK Cluster 1
NK29AG1NK Cluster 2 activation molecule
NK47AG2aNK Cluster 2
FOX5AG1Foxp3
FOX20AG2bFoxP3

Catalog #CloneDescriptionIg isotype
AL-BOV2001H58AMHC IG2a
AL-BOV2003H42AMHC IIG2a
AL-BOV2004TH14BMHC IIG2a
AL-BOV2005TH81A5MHC IIG2a
LT2001LT1AMHC IIG2a
LT2002LT3ACD5 likeG1
LT2003LT4ACD5 likeG2a
LT2004LT97APan TG2b
LT2005LT10ACD2/CD6 likeG2a
LT2006LH43ACD2/CD6 likeG1
AL-BOV2051GB45AWC1-N26G1
AL-BOV2011GC50ACD4M
LT2007LT5ACD8G2a
LT2008LH41ABM
LT2009LT79AB subG3
LT2010LH7AB subM
LT2011LT78AB subM
LT2012LT48ACD11aG2a
LT2013LT36ACD44G2b
LT2014LT41ACD44G2a
LT2015LT12ACD45 likeG2a
LT2016LH9AACT1M
AL-BOV2027CAM36ACD14G1
AL-BOV2029BAQ30ACD18G1
AL-BOV2045TH17ACD47M
AL-BOV2049DH59BCD172aG1

Catalog #CloneDescriptionIg isotype
CT-BOV2001H58AMHC IG2a
CT-BOV2003H42AMHC IIG2a
CT-BOV2004TH14BMHC IIG2a
CT-BOV2005TH81A5MHC IIG2a
CT2012CAT82AMHC IIG1
CT2001CF54APan T1G1
CT2002CF74APan T2G1
CT2003CAT30ACD4G1
CT2003CAT30ACD4G1
CT2015CT1.572 (T572)CD5G2a
CT2013CTOKT8CD8G2a
CT200425-2ACD8G1
CT2014CT3.357 (T357)CD8βM
CT-BOV2021RH1ACD9G3
CT-BOV2027CAM36ACD14G1
CT-BOV2029BAQ30ACD18G1
CT-BOV030HUH82ACD18G2a
CT2005F46ACD21G1
CT2006CL2ACD41 PlateletsG1
CT2007H22ACD44GI
CT-BOV2037BAG40ACD44G3
CT-BOV2049DH59BCD172aG1
CT2008CF26APan lymphocyteG1
CT2011CL35AgranulocyteG1
CT2015CF7AT subG1
CT2016F72AGranulocytes, monocytes, BG1

Catalog #CloneDescriptionIg isotype
BOV2001H58AMHC CL IG2a
BOV2002PT85AMHC CL IG2a
BOV2122B5CMHC CL IG2b
BOV2125W6/32MHC CL IG2a
BOV2120H1AMHC IG2a
BOV2131H6AMHC IG2a
BOV2003H42AMHC CL IIG2a
BOV2004TH14BMHC CL II BoLA-DRG2a
BOV2005TH81AMHC CL II BoLA-DQG2a
BOV2117H34AMHC CL IIG2b
BOV2114BAQ150AMHC IIG3
BOV2115TH16AMHC IIG2a
BOV-CT2012CAT82AMHC IIG1
BOV2007BAQ95ACD2G1
BOV2008MUC2ACD2G2a
BOV211316-1E10CD2G1
BOV2123CH134ACD2G2a
BOV2003H42AMHC CL IIG2a
BOV2004TH14BMHC CL II BoLA-DRG2a
BOV2005TH81AMHC CL II BoLA-DQG2a
BOV2117H34AMHC CL IIG2b
BOV2114BAQ150AMHC IIG3
BOV2115TH16AMHC IIG2a
BOV-CT2012CAT82AMHC IIG1
BOV2007BAQ95ACD2G1
BOV2008MUC2ACD2G2a
BOV211316-1E10CD2G1
BOV2123CH134ACD2G1
BOV2009MM1ACD3G1
BOV2010IL11ACD4G2a
BOV2011GC50ACD4M
BOV2012CACT138ACD4G1
BOV2116CACT83BCD4M
BOV2013B29ACD5G2a
BOV2014CACT105ACD5G1
BOV2015BAQ91ACD6G1
BOV2016BAQ83ACD6G2b
BOV2017CACT80CCD8αG1
BOV2018CACT130ACD8αG3
BOV20197C2BCD8αG2a
BOV2097BAQ111ACD8αM
BOV2020BAT82ACD8βG1
BOV2021RH1ACD9G3
BOV2111B18ACD9G3
BOV2112LT86ACD9G2a
BOV2023HUH73ACD11aG1
BOV2024MM10ACD11bG2b
BOV2025MM12ACD11bG1
BOV2026BAQ153ACD11cM
BOV2066BAQ151ACD11cG1
BOV2124MM31ACD11cM
BOV2027CAM36ACD14G1
BOV2028CAM66ACD14M
BOV2109MM61ACD14G1
BOV2029BAQ30A CD18G1
BOV2030HUH82ACD18G2a
BOV2031GB25ACD21G1
BOV2032BOV2032CD21M
BOV2073CACT108ACD25G2a
BOV2074CACT116ACD25G1
BOV2075GB112ACD25G1
BOV2076LCTB2ACD25G3
BOV2077LCTB32ACD25M
BOV2078CACT114ACD26G2b
BOV2136NK29ACD26G1
BOV2137NK47ACD26G2a
BOV2033TE1ACD28M
BOV2034FW4-101CD29G1
BOV2107ILA158ACD40G1
BOV2036CAPP2ACD41G1
BOV2037BAG40ACD44G3
BOV2038BAT31ACD44G1
BOV2039CACTB51ACD45G2a
BOV2040GS5ACD45RG1
BOV2041GC6ACD45RM
BOV2042ILA116ACD45R0G3
BOV2043GC42ACD45R0G1
BOV2044HUH71ACD47G1
BOV2045TH17ACD47M
BOV2079FW3-218CD49dG2b
BOV2134CACT180ACD50G1
BOV2135CACT216ACD50M
BOV2046BAQ92ACD62LG1
BOV2080KTSN7ACD69G1
BOV2081ILA77ACD71M
BOV2047ILA159CD80G1
BOV2048ILA190ACD86G1
BOV2141LND68ACD163G1
BOV2049DH59BCD172aG1
BOV2105ILA53ACD205G2a
BOV2106ILA114ACD205G1
BOV2133209MD26ACD209G2a
BOV2058GB21ATCR1-N24 d chain specificG2b
BOV2059CACT61ATCR1-N12 d chain specificM
BOV2056CACTB6ATCR1-N6 γ chain specific?M
BOV2057CACTB81ATCR1-N7 γ chain specific?G1
BOV2050B7AWC1+ γδ T cell N1 epitopeM
BOV2053BAQ4AWC1+ γδ T cell N2 epitopeG1
BOV2052GB54AWC1+ γδ T cell N25 epitopeG2a
BOV2054CACTB32AWC1+ γδ T cell N3epitopeG1
BOV2121CACTB7AWC1+ γδ T cell N3 subsetG1
BOV2055BAQ89AWC1+ γδ T cell N4 epitopeG1
BOV2119BAQ159AWC1+ γδ T cell N4 subsetG1
BOV2093BAG2BWC1+ γδ T cell N27 epitopeM
BOV2094BAG25AWC1+ γδ T cell N28 epitopeM
BOV2098CACTB21AWC1+ γδ T cell N29 epitopeG1
BOV2099CACTB31AWC1+ γδ T cell N30 epitopeG2b
BOV2101CACT21AWC1+ γδ T cell N31 epitopeG1
BOV2118ILA29WC1+ γδ T cellG1
BOV2060PIG45AsIgMG2b
BOV2061BIG73AsIgMG1
BOV2062BIG715AIgG1G1
BOV2063BIG501El light chainG1
BOV2064BAQ44AB cellsM
BOV2102GB26AB cellsM
BOV2126ABAQ155AB cells (sIgM?)G1
BOV2067CH138ANeutrophilsM
BOV2068MM20ANeutrophils = CH138AG1
BOV2070BIBab and gd T cellsM
BOV2071TH18APan lymphocyteG3
BOV2072GB50APan leukocyte, endothelial cellsG1
BOV2082CACT200ASLAMF9 (ACT 1)G1
BOV2083CACT206ASLAMF9 (ACT 1)G2a
BOV2092FOX5AFoxp3G1
BOV2103GB53AGr, mono/ lymph subG1
BOV2104GC34ALymphocytes, monocytesM
BOV2132NK64AGp96G1
BOV2138BAQ56APlateletsG1
BOV2139BAQ125APlateletsG2b
BOV2140GC5APlateletsG1

MoAbIg isotypeSpecificity
AV64AG1MHC CL II
AV67AG1αβ TCR?
AV261AG3Pan T
AV66DG3CD4
AV92AMCD4
AV309CG1CD4
AV213AG2aCD8
AV319AG1CD8
AV47AMsIgM
AV191AMLate B
AV306BG1Granulocyte, monocyte
AV243AG2a?Granulocyte, monocyte
AV129AMPan leukocyte
AV14AMPan leukocyte
AV3AMPan leukocyte + RBC
AV18AMPan leukocyte + RBC
AV36AMPan leukocyte + RBC
AV43AG3Pan leukocyte + RBC
AV114AMThrombocyte

Catalog #CloneDescriptionIg isotype
DG-BOV2001MHC IDG-H58AG2a
DG-BOV2003MHC IIDG-H42AG2a
DG-BOV2004MHC IIDG-TH14BG2a
DG-BOV2005MHC IIMHC IIG2a
DG2001DH29ACD4M
DG2002CADO46ACD8G1
DG2003DH3BCD5G1
DG2014DH13ACD5M
DG-BOV2021RH1ACD9G3
DG-BOV2027CAM36ACD14G1
DG-BOV2023HUH73ACD11aG1
DG-BOV2029BAQ30ACD18G1
DG-BOV2030HUH82ACD18G2a
DG-CT2005F46ACD21G1
DG2004DLL20AB and granulocytesM
DG2005DLL51ABG1
DG2006DLL76A BG1
DG2007DLL132ABM
DG-CT2007H22ACD44G2a
DG-BOV2037BAG40ACD44G3
DG-2008CADO18ACD unknown pan leukocyteG1
DG-2009DH16ACD45RBN
DG2010CADO21ACD unknown pan leukocyteG2b
DG2011DH2ACD90M
DG2015DH24ACD90M
DG-BOV2049DH59BCD172aG1
DG-CT2006CL2ACD41G1
DG2012CADO48ANeutrophilsG1
DG2016CADO19ACD45 predictedG1

Catalog #CloneDescriptionIg isotype
G-Bov2001MHC CL I H58AG2a
G-Bov2002MHC CL IPT85AG2a
G-Bov2003MHC CL II HLA-DP likeH42AG2a
G-Bov2004MHC CL II BoLA-DRTH14BG2a
G-Bov2005MHC CL II BoLA-DQTH81AG2a
G-Bov2006CD1bTH97AG2a
G-Bov2007CD2BAQ95AG1
G-Bov2008CD2MUC2AG2a
GT2001GC1ACD4G2a
G-BOV2011GC50ACD4M
GT200217DCD4G1
GT2003BAGB45ACD5G1
G-BOV2015BAQ91ACD6G1
G-BOV2017CACT80CCD8αG1
G-BOV20197C2BCD8αG2a
G-BOV2020BAT82ACD8βG1
G-BOV2023HUH73ACD11aG1
G-BOV2025MM12ACD11bG1
G-BOV2026BAQ153ACD11cM
G-BOV2029BAQ30ACD18G1
G-BOV2031GB25ACD21G1
G-BOV202034FW4-101CD29G1
G-BOV2036CAPP2ACD41G1
G-BOV2027CAM36ACD14G1
G-BOV2028CAM66ACD14M
G-BOV2037BAG40ACD44G3
G-BOV2040GS5ACD45RG1
G-BOV2041GC6ACD45RM
G-BOV2042ILA116ACD45R0G3
G-DG2009DH16BCD45RBG1
G-BOV2045TH17ACD47M
G-BOV2079FW3-218CD49dG2b
GT2010DU1-29CD62LG1
G-BOV2058GB21ATCR1 δ chainG2b
G-BOV2056CACTB6ATCR1-N6 γ chan?M
G-GT201186DTCR1-N6 γ chan?G1
G-BOV2050B7A1WC1-(N1 epitope)M
G-BOV2053BAQ4AWC1-(N2 epitope)G1
G-BOV2054CACTB32AWC1-(N3 epitope)G1
G-BOV2055BAQ89AWC1-( N4 epitope)G1
G-BOV2052GB54AWC1-(N25 epitope)G2a
G-BOV2051GB45AWC1-(N26 epitope)G1
G-BOV2060PIG45A2IgMG2b
G-BOV2062BIG715AIgG1G1
G-BOV2063BIG501Eλ chainG1
G-BOV202064CACT116AB (B-B2)M
G-BOV2074CACT116ACD25G1
G-BOV2075GB112ACD25G1
G-BOV2076LCTB2ACD25G3
G-BOV2038BAT31ACD44G1
G-BOV2012GB26AB cellsM
G-BOV212ILA29WC1G1
GT2012CVR18ALymphocytes monoG1
GT2013BAGB20ACD45 predictedG1

Catalog #CloneDescriptionIg isotype
HM-BOV2003MHC IIH42AG2a
HM2001MHC IIHAT19AG1
HM2002Pan THAT19AG1
HM2003Pan T = HAB2AHAT19AG2a
HM2004HAB1ACD4 predictedG1
HM2005HAL36ACD4 predictedG2a
HM2006HAL17ABG2a
HM2007HASA7ABG1
HM2008HAT3ABM
HM2009HAB6BPan lymphocyteG2a
HM2010HASA18APan lymphocyteM
HM-BOV2029BAQ30ACD18G1
HM2011HASA25ACD45 predictedG1
HM2012HAT13ACD45 predictedG2b

Catalog #CloneDescriptionIg isotype
HR-BOV2001H58AMHC IG2a
HR-BOV2002PT85AMHC IG2a
HR-BOV2003H42AMHC IIG2a
HR-BOV2004TH14BMHC IIG2a
HR-BOV2005TH81A5MHC IIG2a
HRS2001HB88ACD2G1
HRS2002HB61ACD4G1
HRS2003HB65ACD4 subG2a
HRS2004HB86ACD4 subG1
HRS2005HB19ACD5G2a
HRS2006HT23ACD5G1
HRS2007HT14ACD8βG1
HRS2008ETC142B1ACD8αG2a
HRS201773/6.9.1CD8αG3
HR-BOV2023HUH73ACD11aG1
HRS2010H20ACD18G1
HR-BOV2037BAG40ACD44G3
HR-BOV2038BAT31ACD44G1
HRS2011HB32ACD45R0 predictedG2a
HRS2012HB72ACD45R0 predictedG2a
HRS2013E38ACD45R0 predictedG1
HR-DG2009DH16ACD45RBM
HR-BOV2049DH59BCD172aG1
HR-BOV2013B29AB polymorphic (CD5 in bov)G2a
HR-DG2011DH24AGranulocytesM
HRS2016HACT39AGranulocytesG1
HRS20181.9/3.2sIgMG1

Catalog #CloneDescriptionIg isotype
PG200174-11-10MHC CL IG2b
P-BOV2002PG-PT85AMHC CL IG2a
PG2004PG-TH21AMHC IIG2b
PG2005PG-TH22AMHC IIG2a
PG2006MSA3MHC IIG2a
PG-BOV2003PG-H42AMHC IIG2a
PG-BOV2005P-TH81A5MHC II DQ orthologueG2a
PG2007MSA4CD2G2a
PG2008PGBL23ACD2G1
PG2009PG168ACD2G3
PG20108E6CD3G1
PG20118E6-2b3cCD3G2a
PG20128E6-8C8CD3G2a
PG2013PT90ACD4G2a
PG201474-12-4CD4G2b
PG2015PG114ACD5G1
PG2016PG90ACD6G1
PG2017PT36ACD8αG1
PG2018PT81BCD8αG2b
PG201976-2-11CD8αG2a
PG2020PG164ACD8βG2a
PG2021MUC76ACD11aG2a
PG-BOV2027PG-CAM36ACD14G1
PG2022PG-BAQ30ACD18G1
PG-BOV2030PG-HUH82ACD18G2a
PG2023PGBL25ACD25G1
PG2050PG124ACD27 (SWC2)G1
PG-BOV2034PG-FW4-101CD29G1
PG-BOV2037PG-BAG40ACD44G3
PG-BOV2038PG-BAT31ACD44G1
PG202474-9-3A1CD45M
PG2025PG77ACD45RAM
PG2026PG96ACD45RAG1
PG2027PG97ACD45RAM
PG2028PG167ACD45RAG1
PG2029PGB78ACD45RAG2a
PG-BOV2044PG-HUH71ACD47G1
PG-BOV2045PG-TH17ACD47M
PG2030MUC93ACD59G1
PG203174-22-15ACD172a (SWC3)G2b
PG204974-22-15CD172a (SWC3)G1
PG-BOV2049DH59BCD172a (SWC3)G1
PG2032PGBL22ATCR1 δ chain specificG1
PG2033PT14ATCR1 subsetG2a
PG2034PT79ATCR1 subsetG2a
PG2035MUC127ATCR1 subsetG2a
PG2036PG83ASWC4G3
PG2037PG92ASWC5M
PG2038PG94ASubset SWC6+ γδ T cellsM
PG2039PGBL24ASubset γδ T cellsM
PG2040PGBL31ASubset γδ T cellsG1
PG2041PG74CSubsets γδ CD4 CD8 T cellsM
PG2042PG104ACD8+ CD4 sub, γδ small subM
PG204376-7-4B cell and thymocytesG2a
PG2045PG68AGranulocytesG1
PG2046PG145AsIgMM
PG-BOV2060PIG45A2sIgMG2b
PG2047PG89APan lymphocyteG1
PG2048PG106APan lymphocyteM

Catalog #CloneDescriptionIg isotype
R-BOV2001R-H58AMHC IG2a
R-BOV2004R-TH14BMHC II G2a
RB2001RTH2AT1G1
RB2002RTH21AT2G1
RB2003RT22AT2 blocked RTH21AM
RB2004MRB61AT3G1
RB2005RTH26AT4 = ken5G2a
RB2006RTH1ACD4G1
RB2007RTH192ACD5G1
RB2008ISC16ACD8M
RB2009ISC27ACD8G2a
RB2010ISC29ECD8G1
RB2011ISC38ACD8G1
R-BOV2021RH1ACD9G3
R-BOV2023HUH73ACD11aG1
RB2021RT18ACD11bM
R-BOV2027CAM36ACD14G1
R-BOV2029BAQ30ACD18G1
R-BOV2030HUH82ACD18G2a
R-BOV2037BAG40ACD44G3
RB2022ISC18ACD45G2a
RB2023ISC39ACD45G1
RB2024ISC76ACD45M
RB2025RTH32ACD58M
RB2026RTH33ACD58G1
RB2012RACT19ACD4 CD8 SUB CD28?M
RB2013RACT20ABasophils and sub CD4 CD8G1
RB2014MRB120AGranulocytes, basophils, monocytesG1
RB2015MRB25ABM
RB2031MRB143ABM
R-BOV2064BAQ44ABAQ44AM
RB2017RT19AB subM
RB2018MRB107AB subG1
RB2019MRB102APan lymphocyteM
RB2020RTH186APan lymphocyteG1
RB2027RACT44AGranulocytesM
RB2028RACT38APan leukocyteG1

Catalog #CloneDescriptionIg isotype
S-BOV2001H58AMHC CL IG2a
S-BOV2002PT85AMHC CL IG2a
S-BOV2003H42AMHC CL IIG2a
S-BOV2004TH14BMHC CL II BoLA-DRG2a
S-BOV2005TH81AMHC CL II BoLA-DQG2a
S-BOV2006TH97ACD1bG2a
S-BOV2008MUC2ACD2G2a
S-GT2001GC1ACD4G2a
S-BOV2011GC50ACD4M
S-GT200217DCD4G1
SHP2001ST1ACD5IgG2a
SHP2002ST8CD8M
S-BOV2017CACT80CCD8αG1
S-BOV20197C2BCD8αG2a
S-BOV2020BAT82ACD8βG1
S-BOV2023HUH73ACD11aG1
S-BOV2025MM12ACD11bG1
S-BOV2026BAQ153ACD11cM
S-BOV2029BAQ30ACD18G1
S-BOV2034FW4-101CD29G1
S-BOV2036CAPP2ACD41G1
S-BOV2027CAM36ACD14G1
S-BOV2028CAM66ACD14M
S-BOV2037BAG40ACD44M
S-BOV2040GS5ACD45RG1
S-BOV2041GC6ACD45RM
S-BOV2042ILA116ACD45R0G3
S-DH2009DH16BCD45RBM
S-BOV2045TH17ACD47M
S-BOV2079FW3-218CD49dG2b
S-GT2010DU1-29CD62LG1
S-BOV2056CACTB6ATCR1-N6 γ chain ?M
S-GT201186DTCR1 γ chain ?G1
S-BOV2050B7A1WC1-N1M
S-BOV2053BAQ4AWC1-N2G1
S-BOV2121CACTB7AWC1-N3G1
S-BOV2055BAQ89AWC1-N4G1
S-BOV2052GB54AWC1-N25G2a
S-BOV2051GB45AWC1-N26G1
S-BOV2060PIG45A2sIgMG2b
S-BOV2062BIG715AG1G1
S-BOV2063BIG501Eλ chainG1
S-BOV2064BAQ44AB (B-B2)M
S-BOV2074CACT116ACD25G1
S-BOV2075GB112ACD25G1
S-BOV2076LCTB2ACD25G3
S-CT2012CAT82AMHC IIG1

MoAbIg isotypeSpecificity
PT85AG2aMHC Cl I
H58AG2aMHC Cl I
H42AG2aMHC Cl II
TH14BG2aMHC Cl II
TH81A5G2aMHC Cl II
BAQ95AG1CD2
MUC2AG2aCD2
MM1AG1CD3
CACT138AG1CD4
GC50A1MCD4
ILA11AG2aCD4
BAQ91AG1CD6
CACT80CG1CD8α
CACT88CG3CD8α?
7C2BG2aCD8α?
TH82AG1CD8β?
TH2AG2aCD9
B18AG3CD9
HUH73AIgG1CD11a
MM12AG1CD11b
BAQ153AMCD11c
CAM36AG1CD14
BAQ30AG1CD18
HUH82AIgG2aCD18
GB25AG1CD21
CACT116AG1CD25
LCTB2AG3CD25
GB112AG1CD25
CACT114AG2bCD26
BAT31AG1CD44
LT36AG2bCD44
LCT27AG1CD45R
ILA116AG3CD45R0
TH17AMCD47
BAQ92AG1CD62L
DH59BG1CD172a
GB21A1G2bγδ TCR-N24 δ chain
CACTB6AMγδ TCR-N6 γ chain?
CACTB81AG1γδ TCR-N7 γ chain?
CACTB44AG1γδ TCR-N7 CL
B7A1MWC1-N1
CGB24AG1WC1-N1 CL
BAQ4AG1WC1-N2
CACTB32AG1WC1-N3
BAQ89AG1WC1-N4
BAQ159AG1WC1-N4 CL
CACTB31AG2bWC1-N22
GB54AG2aWC1-N25
GB45AG1WC1-N26
PIG45A2G2bIgM
BIG501EG1λ light chain
BAQ44AMB-B2
GB26AMB + T sub
LND37AG1M
LND68AG1M
CH138AMGr + endo
MM20AG1Gr + endo = CH138A
CACT200AG1ACT1
CACT206AG2aACT1
CACT298AG2aACT1
LCTB6AG1ACT9
GB110AMACT16
CACT216AMACT28
CACT225AG1ACT29
CACT152AMACT31
CACT191AMACT32
CACT180AG1ACT34
CACT282AMACT35
CACT153AMACT37
CACT243AMACT38

About us

In the mid-70s, a group of investigators demonstrated in mice that it is possible to immortalize B lymphocytes producing single monospecific antibodies (mAbs) by fusing B lymphocytes to tissue culture adapted myeloma (malignant form of an antibody producing cell) cells. The hybrid cells (hybridomas) were stable and could be maintained in culture indefinitely or preserved in liquid nitrogen for later use. These findings represented a major breakthrough in the way antibody reagents could be developed for basic and biomedical research. Mice could be immunized with antigens of interest and then used as a source of B lymphocytes to produce mAbs of known specificity. The spleen was shown to be the best source of B lymphocytes for making hybridomas. Hybridomas producing the desired mAbs could be identified by examining culture medium from primary cultures of fused cells. In subsequent investigations, myeloma fusion partners were developed that could only grow in selective growth medium if fused to a B lymphocyte, eliminating the problem of distinguishing hybridomas from cells that had not fused with a B lymphocyte. Myeloma fusion partners were later identified that could no longer produce their own antibody but could produce antibody derived from the B lymphocyte. These advances simplified and improved the methodology for producing mAbs.

Research universities, research institutes and industry established core facilities to exploit mAb technology to develop mAbs for use as analytical tools in the biological and biomedical sciences, in the characterization of the immune system, study the mechanisms by which pathogens cause disease, develop improved diagnostic assays, and identify antigens from pathogens that can be used in subunit vaccines. Because of our research needs, we started developing mAbs for use in food and companion animal research and diagnostic medicine in 1979. To encourage other investigators to use mAb technology, we sponsored the first international symposium and workshop ‘Impact of Monoclonal Antibody Technology on Animal and Plant Agricultural Research’ in June of 1982. We also participated in a workshop sponsored by the National Research Council of the National Academy of Science on ‘Priorities in Biotechnology Research for International Development held in July of 1982, a symposium sponsored by the USDA ‘Hybridoma Technology in Agricultural and Veterinary Research’ held in Oct. of 1983 and an international conference sponsored by the International Laboratory for Research on Animal Diseases ‘The Ruminant Immune System In Health and Disease’ held in Kenya Africa in September 1983. In 1984, we co-sponsored a meeting with Australia ‘Characterization of the Bovine Immune System and the Genes Regulating Expression of Immunity with Particular Reference to their Role in Disease Resistance’ held in Honolulu, Hawaii. During this period of time, we developed some of the first sets of mAbs for use in the study of the immune system in food and companion animals. We also participated in all the international workshops to characterize the first sets of mAbs.

Goals

The Washington State University Monoclonal Antibody Center was established in 1982 with the following goals:

  1. Provide a central facility for the production of mAbs for investigators that do not have the facilities or expertise to produce mAbs for their research.
  2. Develop and use mAbs to study the mechanisms regulating the immune response to pathogens and candidate subunit vaccines in veterinary species.
  3. Provide a source of mAbs for the research community for use in food and companion animal research.
  4. Provide graduate and professional training in the use of mAb technology in research and the development of diagnostic reagents.
  5. Provide graduate and professional training in the use of mAbs in immunology and flow cytometry.
  6. Collaborate with investigators requiring expertise and resources available through the center to conduct studies in immunology.
  7. Aid in the development of clinical flow cytometry diagnostic services in veterinary medicine.